Multilocus enzyme electrophoresis analysis ofEchinostoma revolutumand Echinostoma malayanum (Tremato

• 作 者: Weerachai Saijuntha Sumonta Tapdara Chairat Tantrawatpan
• 机 构: Walai Rukhavej Botanical Research Institute WRBRI, Mahasarakham University, Mahasarakham, Thailand“,
• 出 处: 亚太热带医药杂志（英文版）
• 发表日期: 2010年8期
• 关键词: Echinostoma Echinostomes Genetic variation Multilocus enzyme electrophoresis All

Objective:To explore the genetic variation and differentiation of 2 echinostomes from genus Echinostoma, i.e.Echinostoma revolutum (E. revolutum)andEchinostoma malayanum (E. malayanum) from Khon Kaen Province, Thailand.Methods: These parasites were compared at22enzymes encoding a presumptive30 loci by using multilocus enzyme electrophoresis (MEE) technique.Results: Twenty-two loci can be used as diagnostic markers to differentiate these2species.E. revolutum andE. malayanum had fixed genetic differences at70% of loci, whereas both species had fixed genetic differences from the liver fluke,Opisthorchis viverrini at91% of loci. Intraspecific variation within a population ofE. revolutum was observed at5 polymorphic loci.Conclusions:MEE is a powerful technique to investigate genetic variation and differentiation ofE. revolutum andE. malayanum.