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本试验主要探讨了紫花苜蓿根结线虫病(Meloidogyne arenaria Chituood)的发病温度及从接种到病情调查所需的培养时间等,并对9个紫花苜蓿品种进行了抗病性评价。结果表明,M.are(?)ia在紫花苜蓿上的最适发病温度为29℃,但抗病性鉴定筛选可在25～33℃之间进行。线虫接种到病情调查的最适培养期为80天。抗病性鉴定中发现源于沙特阿拉伯的Hasawi NE—NAF—4和Hasawi SA19两品种对M.arenaria表现高抗,其抗病植株所占比例分别达80.7%和81.8%,可作为今后紫花苜蓿抗M.arenaria育种的抗源。美国品种UC Cibola、UC297和UC 298亦表现了良好的抗病性,抗病植株分别占鉴定苗数的58.9%、60.3%和65.8%。原产埃及的两个品种EgyptianIV和Egyptian II感病最重,它们的感病植株所占比例分别高达89.5%和93.3%。
The experiment mainly discussed the onset temperature of Meloidogyne arenaria Chituood and the culture time from inoculation to disease investigation. The resistance of nine alfalfa cultivars was evaluated. The results showed that the optimum temperature of M.are (?) Ia on alfalfa was 29 ℃, but the identification of disease resistance could be carried out at 25 ~ 33 ℃. Nematode inoculation to disease investigation of the optimal culture period of 80 days. In the resistance evaluation, two species of Hasawi NE-NAF-4 and Hasawi SA19 from Saudi Arabia were found to be highly resistant to M.arenaria, and their resistant plants accounted for 80.7% and 81.8% of the total, respectively, which could be used as future alfalfa Resistance to M.arenaria breeding. The American varieties UC Cibola, UC297 and UC 298 also showed good disease resistance, and the resistant plants accounted for 58.9%, 60.3% and 65.8% of the identified seedlings respectively. EgyptIV and Egyptian II, two Egyptian origin breeds, were the most susceptible. Their susceptible plants accounted for as much as 89.5% and 93.3% respectively.