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目的了解安阳市健康人群中肠道病毒隐形感染的情况及特征,比较不同样品类型、PCR检验方法对检测结果的影响。方法选取安阳市滑县的一个自然村作为调查点。根据年龄梯度共选取100人作为调查对象。每人采集粪便及咽拭子标本各一份。采用Realtime-PCR和普通RT-PCR两种方法检测咽拭子和粪便样品中的肠道病毒及型别。结果 2010年肠道病毒隐性感染人数为36人,隐性感染率为36.00%;2011年肠道病毒隐性感染人数为35人,隐性感染率为35.00%。其中非EV71和CA16的其它型肠道病毒感染人数最多,其次是EV71型。5岁以下年龄组肠道病毒感染率最高。粪便样品较咽拭子样品的阳性检出率高,Realtime-PCR较普通RT-PCR敏感性更好。结论肠道病毒隐性感染在健康人群中具有较高的比例,对手足口病防治提出了新的挑战。肠道病毒的实验室检测推荐使用Realtime-PCR和粪便标本,可获得更为准确的检测结果。
Objective To understand the situation and characteristics of the intestine virus invisible infection in healthy population in Anyang City and compare the effects of different sample types and PCR test methods on the test results. Methods A natural village in Huaxian County, Anyang City was selected as the investigation point. A total of 100 people were selected as the survey subjects according to the age gradient. Each person collected a sample of feces and throat swabs. Realtime-PCR and RT-PCR were used to detect enterovirus and its type in throat swabs and stool samples. Results The number of latent infections of enterovirus in 2010 was 36, and the latent infection rate was 36.00%. In 2011, the number of latent infections of enteroviruses was 35, and the latent infection rate was 35.00%. Among them, non-EV71 and CA16 had the highest number of other enterovirus infections, followed by EV71. The highest infection rate of enterovirus was found in the group under 5 years old. The positive detection rate of stool samples was higher than that of throat swab samples, Realtime-PCR was more sensitive than ordinary RT-PCR. Conclusion The latent infection of enteroviruses has a higher proportion in healthy population, posing new challenges for the prevention and treatment of foot-mouth disease. Laboratory testing for enteroviruses Realtime-PCR and stool specimens are recommended for more accurate detection.