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目的:建立测定窝儿七中3种木脂素含量的方法。方法:采用HPLC法,色谱柱为Cosmosil-C18(4.6 mm×250 mm,5μm),柱温为25℃,甲醇-0.4%磷酸溶液梯度洗脱,流速为1 m L·min-1,检测波长为330 nm。结果:4’-去甲基鬼臼毒素、鬼臼毒素和山荷叶素分别在4.5~224.0μgm L·L-1(r=0.9992)、20.0~1000.0μg·m L-1(r=0.9994)和5.1~256.2μg·m L-1(r=0.9994)线性关系良好,平均回收率分别为99.80%(RSD%=1.44%)、99.82%(RSD%=1.67%)和99.28%(RSD%=2.30%)。结论:所建立的方法灵敏、准确、重复性好,为窝儿七的质量控制提供参考依据。
Objective: To establish a method for determination of three kinds of lignans in nest child seven. Methods: The chromatographic column was Cosmosil-C18 (4.6 mm × 250 mm, 5 μm) with a gradient elution of methanol-0.4% phosphoric acid at 25 ℃ and a flow rate of 1 m L · min-1. The detection wavelength Is 330 nm. RESULTS: The results showed that 4’-demethylation of podophyllotoxin, podophyllotoxin and okamotin were respectively in the range of 4.5-224.0μgm L · L -1 (r = 0.9992), 20.0-1000.0μg · m L -1 (r = 0.9994 (RSD% = 1.44%), 99.82% (RSD% = 1.67%) and 99.28% (RSD%), respectively, with 5.1 ~ 256.2μg · m L -1 (r = 0.9994) = 2.30%). Conclusion: The established method is sensitive, accurate and reproducible, which provides a reference for the quality control of