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目的:探讨四神丸治疗溃疡性结肠炎(UC)的作用机制。方法:将40只SD大鼠随机分为正常组、模型组、对照组及四神丸组各10只。除正常组外,其余各组均采用2、4、6-三硝基苯磺酸(TNBS)/乙醇法复制UC大鼠模型,灌胃14 d后观察各组大鼠血清TLR4、TGF-β1、IL-10的变化。结果:模型组大鼠血清TLR4水平增高,TGF-β1、IL-10水平降低,与正常组比较,差异均有统计学意义(P<0.01);四神丸组TLR4、TGF-β1、IL-10均较模型组明显改善(P<0.05或P<0.01)。结论:四神丸可调节溃疡性结肠炎大鼠TLR4-DC免疫耐受失调,可能是其治疗UC的作用机制之一。
Objective: To explore the mechanism of action of Si Shen Pill in the treatment of ulcerative colitis (UC). Methods: Forty SD rats were randomly divided into normal group, model group, control group and Si Shen Pang group. In addition to the normal group, the rats in each group were treated with 2,4,6-trinitrobenzene sulfonic acid (TNBS) / ethanol for UC rat model. After 14 days of intragastric administration, the expressions of TLR4, TGF-β1 , IL-10 changes. The levels of TLR4, TGF-β1 and IL-10 in the model group were significantly higher than those in the normal group (P <0.01) 10 than the model group was significantly improved (P <0.05 or P <0.01). Conclusion: Si Shen Pill can regulate the imbalance of TLR4-DC immune tolerance in rats with ulcerative colitis, which may be one of the mechanisms of its treatment of UC.