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目的和方法：本文利用原位杂交和点杂交的方法，对ＴｒｋＣｍＲＮＡ在大鼠脊髓组织中的分布与坐骨神经损伤后的表达变化进行了研究。结果：ＴｒｋＣｍＲＮＡ几乎存在于所有脊髓神经元与胶质细胞中，在坐骨神经损伤后１ｄ表达增加，以后降到较低水平，到１４ｄ又再次升高，但第二峰较第一峰为低。ＴｒｋＣｍＲＮＡ在大鼠脊髓组织中的广泛分布，表明ＮＴ３对脊髓组织各类神经元均有可能的神经营养作用。坐骨神经损伤后１ｄ表达的增加，可能与损伤应激相关，１４ｄ的再次升高可能由于再生神经渐达靶组织所致。结论：受体表达的改变，可能是受损神经元在损伤与再生修复过程中，神经元本身代谢适应性改变的一部分
Objective and Methods: In this study, the distribution of TrkC mRNA in rat spinal cord tissue and the expression after sciatic nerve injury were studied by in situ hybridization and dot blot hybridization. Results: TrkC mRNA was found in almost all spinal cord neurons and glial cells. The expression of TrkC mRNA was increased at 1 day after sciatic nerve injury, then decreased to a lower level and then increased again at 14 days. However, the second peak was lower than the first peak. The wide distribution of TrkC mRNA in rat spinal cord tissue suggests that NT3 may have neurotrophic effects on various types of neurons in spinal cord tissue. The increased expression of sciatic nerve 1d after injury may be related to injury stress. The re-elevation of 14d may be caused by the regenerating nerve gradually reaching the target tissue. CONCLUSIONS: The altered expression of receptors may be part of the altered adaptive fitness of neuronal cells during the course of injury and regenerative repair of damaged neurons