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目的探讨CC趋化因子配体1(CCL1)的3个基因位点rs159291、rs159294和rs210837与T2DM并发肺结核(T2DM-PTB)发病风险的关系。方法收集单纯T2DM者(T2DM组)124例、T2DM伴PTB者(T2DM-PTB组)124例及健康者(NC组)130名。采用病例对照法,运用RT-PCR对3个基因位点进行基因分型,分析各组基本特征和基因分型结果。结果各组性别、年龄、BMI、TG、TC比较,差异无统计学意义,且T2DM组和T2DM-PTB组FPG比较,差异无统计学意义[(11.35±3.54)vs(11.56±4.22)mmol/L,P>0.05];3组rs159291位点的AA、AG和GG基因型频率分布比较,差异有统计学意义(P<0.01),3组间两两比较结果显示,基因型AA可增加T2DM-PTB的发病风险(P<0.01)。其他两个位点在3组的基因型频率分布比较,差异无统计学意义(P>0.05)。结论 rs159291的AA基因型是T2DM-PTB的易感基因型,rs159294、rs210837位点与T2DM-PTB风险无关。
Objective To investigate the relationship between rs159291, rs159294 and rs210837 and the risk of T2DM complicated with pulmonary tuberculosis (T2DM-PTB) in CCL1 gene. Methods 124 patients with T2DM (T2DM group), 124 patients with T2DM with PTB (T2DM-PTB group) and 130 healthy people (NC group) were collected. The case-control method was used to genotype the three gene loci by RT-PCR, and the basic characteristics and genotyping results of each group were analyzed. Results There was no significant difference in gender, age, BMI, TG and TC among the two groups (P <0.05), but there was no significant difference in FPG between T2DM group and T2DM-PTB group (11.35 ± 3.54 vs 11.56 ± 4.22 mmol / L, P> 0.05]. The frequencies of AA, AG and GG genotypes in rs159291 were significantly different among 3 groups (P <0.01). The results of pairwise comparisons showed that genotype AA increased T2DM The incidence of PTB (P <0.01). There was no significant difference in genotype distribution between the other two sites in the three groups (P> 0.05). Conclusion AA genotype of rs159291 is a susceptible genotype of T2DM-PTB. Rs159294 and rs210837 sites have no relationship with T2DM-PTB risk.