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[目的]分析复发鼻咽癌和初发鼻咽癌的蛋白质磷酸化表达差异,以期为复发鼻咽癌的发生机制提供分子依据。[方法]选择4例复发鼻咽癌患者和4例初发鼻咽癌患者的组织标本,实验室提取总蛋白,应用磷酸化抗体蛋白质芯片与提取蛋白进行杂交,检测两者之间的差异磷酸化位点。通过phosphosite在线网络数据库分析蛋白质磷酸化位点变化及在信号通路中的作用。[结果]复发鼻咽癌和初发鼻咽癌的蛋白质磷酸化表达不同,共筛选出16个差异磷酸化位点,15个差异蛋白,复发鼻咽癌组织中上调蛋白8个,下调蛋白7个。[结论]初发鼻咽癌和复发鼻咽癌组织之间存在蛋白质磷酸化水平差异,可能与鼻咽癌复发机制有关。
[Objective] To analyze the difference of protein phosphorylation between recurrent nasopharyngeal carcinoma and nasopharyngeal carcinoma, and to provide a molecular basis for the pathogenesis of recurrent nasopharyngeal carcinoma. [Method] Tissue samples from 4 patients with nasopharyngeal carcinoma and 4 patients with nasopharyngeal carcinoma were selected. The total protein was extracted from the laboratory and the phosphoprotein was detected by hybridization with the extracted protein. The difference between the two The site. Analysis of changes in phosphorylation sites and their roles in signaling pathways via phosphosite online web database. [Results] The protein phosphorylation of nasopharyngeal carcinoma and nasopharyngeal carcinoma were different. A total of 16 differentially phosphorylated sites and 15 differentially expressed proteins were identified, including up-regulated protein 8 and down-regulated protein 7 in nasopharyngeal carcinoma A [Conclusion] The difference of protein phosphorylation between nasopharyngeal carcinoma and recurrent nasopharyngeal carcinoma may be related to the recurrence mechanism of nasopharyngeal carcinoma.