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本文研究了食品污染物棒曲霉毒素诱导人胚肾细胞损伤过程中基因表达谱动态变化。5μM PAT作用HEK293细胞3、24h,应用数字基因表达谱(DGE)技术筛选差异表达基因,分析基因富集的GO功能,KEGG信号通路及Pathway信号途径分析,并通过定量PCR对部分差异表达基因进行验证。分析发现,在3 h和24 h受PAT诱导的差异表达基因分别为79个和261个,其中上调表达基因分别为71和192个,下调表达基因分别为8和69个。PAT主要诱导了细胞凋亡基因的差异表达,RNA降解差异基因全部表达上调,RNA合成差异基因表达下调,泛素介导的蛋白降解大部分差异基因上调,MAPK信号通路主要参与PAT毒性的下游进程,其相关差异基因大部分上调。DGE技术为我们在分子水平上提供了PAT胚肾细胞损伤机制的众多线索,为相关基因生物学效应研究奠定基础。
In this paper, the dynamic changes of gene expression profile during human embryonic kidney cell injury induced by food contaminants of patulin were studied. HEK293 cells were treated with 5μM PAT for 3 and 24 hours, respectively. The differentially expressed genes were screened by digital gene expression profiling (DGE), and the GO enrichment, KEGG signaling and Pathway signaling pathways were analyzed by quantitative polymerase chain reaction (PCR) verification. The results showed that there were 79 and 261 differentially expressed genes induced by PAT at 3 h and 24 h, respectively, with 71 and 192 up-regulated genes, and down-regulated genes were 8 and 69, respectively. PAT mainly induced the differential expression of apoptotic genes, all of them were upregulated in RNA degradation genes, down-regulated in RNA synthesis and up-regulated in ubiquitin-mediated protein degradation. MAPK signaling pathway was mainly involved in the downstream process of PAT toxicity , Most of the related genes were up-regulated. DGE technology provides many clues to the mechanism of PAT embryonic kidney cell damage on the molecular level and lays the foundation for the study of biological effects of related genes.