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目的:观察维甲酸(RA)体外诱导神经干细胞后体内移植治疗失神经肌萎缩的效果。方法:共使用成年SD大鼠28只,切断两侧坐骨神经,随机选择一侧直接吻合坐骨神经作为对照组,实验组从孕龄15d的胚胎SD大鼠脊髓组织中分离获得脊髓源性神经干细胞,经维甲酸(RA)体外诱导后,显微注射到SD大鼠失神经支配的一侧踇长伸肌中,术后16周神经电生理和免疫组织化学等方法观察。结果:实验组与对照组间的踇长伸肌肌肉纤颤电波幅度,神经肌纤维传导速度的数目无统计学差异(P>0.05)。结论:神经干细胞经体外诱导后体内移植具有治疗失神经肌萎缩的作用。
Objective: To observe the effect of retinoic acid (RA) on neural stem cells transplantation in vivo for the treatment of denervated atrophy. Methods: A total of 28 adult Sprague-Dawley rats were used to cut off bilateral sciatic nerves. One side of the sciatic nerve was randomly selected as the control group. Spinal cord-derived neural stem cells were obtained from spinal cord of embryonic SD rats 15 days old. After induction of retinoic acid (RA) in vitro, it was microinjected into the long extensor muscle of the denervated dorsal horn of SD rats and observed by electrophysiological and immunohistochemical methods 16 weeks after operation. Results: There was no significant difference in the amplitude of muscle fibrillation and the number of nerve fiber conduction between the experimental group and the control group (P> 0.05). Conclusion: The neural stem cells can be transplanted into the body in vitro for the treatment of denervated muscular atrophy.