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目的观察其海马经HE染色后组织病理学、胶质纤维酸性蛋白免疫反应阳性表达细胞在LPS中各观察时间点海马CA1、CA3、齿状回的表达,探讨其致机制。方法锂-匹罗卡品急性诱导SD癫持续状态模型鼠形成后,采用免疫组化和图像分析方法观察海马HE染色组织病理学、胶质纤维酸性蛋白免疫反应阳性表达细胞。结果模型组各时间点海马细胞形态出现病理性改变,部分细胞脱失,胞浆浓缩,胞核固缩深染;胶质纤维酸性蛋白免疫反应阳性表达细胞亦显著上调(P<0.05)。结论Pilo诱导SD大鼠癫发作后存在显著的海马神经元结构和胶质细胞的损伤,以胶质细胞损伤更显著,胶质纤维酸性蛋白持续高表达可能是这种功能异常的胶质细胞增生的重要原因,也可能是锂-匹罗卡品致癫发作的重要因素之一。
Objective To observe the expression of hippocampal CA1, CA3 and dentate gyrus in hippocampus after HE staining and the expression of glial fibrillary acidic protein immunoreactive cells in hippocampus of LPS. Methods Immunohistochemistry and image analysis were used to observe the histopathology and glial fibrillary acidic protein immunoreactive positive cells in hippocampus of rats induced by lithium-pilocarpine acutely induced SD epilepticus. Results At each time point, the morphology of hippocampal cells in the model group showed pathological changes, part of the cells were lost, the cytoplasm was concentrated, and the cytoplasm was deeply condensed. The glial fibrillary acidic protein immunoreactive positive cells were also significantly up-regulated (P <0.05). Conclusion Pilo induced significant damage of hippocampal neurons and glial cells after epileptic seizures in SD rats, especially in glial cells. The sustained high expression of glial fibrillary acidic protein may be the abnormal glial cells An important reason for hyperplasia, may also be one of the important factors of epileptic seizures caused by lithium-pilocarpine.