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目的：比较API和VITEK2法鉴别VanC型肠球菌的准确性。方法针对2005～2011年收集的50株VanC型肠球菌，采用API和VITEK2方法进行种属鉴定，结合细菌动力实验，用分子生物学方法做对照，以判断API和VITEK2鉴定结果的准确性。结果 API和VITEK2方法分别筛选出VanC型肠球菌21，15株，与分子生物学方法比较，其准确率分别为28.6％，86.7％，结合动力实验结果后，其准确率均达到100％。结论 VITEK2比API在诊断VanC型肠球菌时具有更好的准确性，2种方法联合动力实验可为临床提供准确的细菌学诊断。“,”Objective To compare the API and VITEK2 in identifying VanC type Enterococcus.Method A total of 50 VanC type Entero-coccus isolates from 2005~2011 were investigated.We performed the API and VITEK2 test to identified the VanC type Enterococcus respec-tively.API and VITEK2 results combining with the motility test was compared with molecular biology methods to determine the accuracy of API and VITEK2.The MIC of vancomycin and tecoplanin for the strains was performed by agar dilution method.Results Twenty -one and 15 strains of VanC phenotype Enterococcus were identified by the API and VITEK2 test respectively.Compared with molecular bio-logy methods, the accuracy rate was 28.6% and 86.7% respective-ly.Combined with motility test, the accuracy rate of API and VITEK2 reached 100%.Conclusion The VITEK2 had a higher accuracy rate in identifying VanC phenotype Enterococcus than API.API/VITEK2 method combined with bacterial motility test can provide an accurate bacteriological diagnosis for the patients.