论文部分内容阅读
通过白菜型油菜‘R-O-18’和菜薹‘L58’的基因组重测序数据与白菜基因组的参考序列(‘Chiifu-401-42’的基因组序列)的比对,在全基因组范围内检测到了18479个短插入缺失变异位点(≤5bp)。从中挑选了500个插入/缺失片段为4～5bp的InDel变异位点,将其设计成InDel分子标记并进行试验验证,结果有452个标记通过PCR扩增出单一条带,但仅有106个在‘R-O-18’和‘L58’间表现出多态性,346个没有多态性,48个在PCR中没有扩增。亲本间具有多态性的106个InDel标记可用来检测以‘R-O-18’和‘L58’为亲本构建的RILs基因型,并构建了一张包含99个标记的遗传连锁图谱。
Through genome-wide re-sequencing of the genomes of cabbage rape ’RO-18’ and ’Cai-sprout’ L58 ’and the reference sequence of the Chinese cabbage genome (’ Chiifu-401-42 ’), 18479 A short insertion mutation site (≤ 5bp). 500 InDel mutated sites with 4 ~ 5bp insertions / deletions were selected and designed as InDel molecular markers and tested. As a result, 452 markers were amplified by PCR and only 106 bands There were polymorphisms between ’RO-18’ and ’L58’, 346 with no polymorphism and 48 without amplification in PCR. Sixty-six InDel markers with polymorphism were used to detect the RILs genotypes with ’R-O-18’ and ’L58’ as parents and a genetic linkage map with 99 markers was constructed.