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目的 观察不同浓度参麦注射液预处理对肢体缺血再灌注肺损伤(LIRLI)后血浆炎症因子和肺损伤的影响.方法 用大鼠双下肢缺血3h再灌注4h制备LIRLI模型.按照随机数字表法将SD大鼠分为5组(n=10):假手术组,模型组和低、中、高3个剂量实验组(参麦注射液1,2,3 mL · kg-1预处理+LIRLI).假手术组与模型组用等量0.9％ NaCl输注;在实施肢体缺血再灌注前10 min,实验组将相应剂量的参麦注射液加入0.9％ NaCl 20 mL静脉滴注.实验结束后,进行血气分析,以酶联免疫吸附法检测血浆中炎症因子浓度,以苏木精伊红(HE)染色后显微镜下进行肺损伤评分.结果 假手术组、模型组和低、中、高3个剂量实验组的pH值分别为7.35±0.09,7.15±0.08,7.25 ±0.11,7.31±0.10,7.36±0.14,与假手术组比较,模型组差异有统计学意义(P＜0.05);与模型组比较,高剂量组差异有统计学意义(p＜0.05).与假手术组比较,模型组PaO2值明显降低,PaCO2显著升高,差异均有统计学意义(均P＜0.05).低、中、高3个剂量实验组的PaO2值明显高于模型组、而PaCO2显著低于模型组,差异均有统计学意义(均P＜0.05).假手术组、模型组、低、中、高3个剂量实验组的肿瘤坏死因子(TNF-α)分别为(1.37±0.15),(6.78±1.23),(5.28 ±0.79),(3.35±0.32),(2.31±0.25) nmol·mL-1,与假手术组比较,模型组差异有统计学意义(P＜0.05);与模型组比较,低、中、高3个剂量实验组差异均有统计学意义(均P＜0.05).假手术组、模型组和低、中、高3个剂量实验组的白细胞介素(IL)-6与IL-10分别为(160±24),(342±34),(324±29),(289±33),(253±37) pg.mL-1;(175±12),(120±14),(132±6),(145±11),(155 ± 15) pg·mL-1,与假手术组比较,模型组差异均有统计学意义(均P＜0.05);与模型组比较,低、中、高3个剂量实验组差异均有统计学意义(均P ＜0.05).假手术组、模型组和中、高2个剂量实验组的肺损伤评分(分)分别为0.75±0.23,7.26±0.52,3.65 ±0.32,1.39±0.25,与假手术组比较,模型组差异有统计学意义(P＜0.05):与模型组比较,中、高2个剂量实验组差异均有统计学意义(均P ＜0.05).结论 参麦注射液预处理通过减少大鼠肺部炎症因子释放,从而有效地减轻LIRLI.“,”Objective To observe the effects of Shenmai injection on plasma inflammatory cytokines and lung injury in limbs ischemia-reperfusion lung injury (LIRLI)of rats.Methods SD rats with LIRLI were randomly divided into five groups (n =10):Sham operation group,model group,experimental-L,experimental-M,experimental-H groups (1,2,3 mL · kg-1 Shenmai injection).Blood gas analysis was performed at the end of the experiment.The enzyme linked immunosorbent assay was used to measure the concentration of inflammatory cytokines in the plasma.The lung injury score was evaluated with hematoxylin eosin(HE) by microscope.Results pH value in sham operation group,model group,experinental-L,experimental-M,experimental-H groups were 7.35 ± 0.09,7.15 ± 0.08,7.25 ± 0.11,7.31 ± 0.10,7.36 ±0.14.pH value in model group was lower than sham operation group with significantly while experimental-H group was higher than model group significantly (all P ＜ 0.05).Compared sham operation group,PaO2 in model group were significantly lower and PaCO2 was significantly higher(all P ＜ 0.05).Compared with model group,PaO2 in three doses experimental groups were significantly higher and PaCO22 in three doses experimental groups was significantly lower(all P ＜ 0.05).Tumor necrosis factor-α (TNF-α) in sham operation group,model group,experimental-L,experimental-M,experimental-H groups were (1.37 ± 0.15),(6.78 ± 1.23),(5.28 ± 0.79),(3.35 ± 0.32),(2.31 ±0.25) nmol · mL-1.Compared with sham operation group,TNF-α in model group were significantly higher (P ＜ 0.05).Compared with model group,TNF-α in three doses experimental groups were significantly lower (all P ＜0.05).Interleukin(IL)-6 and IL-10 in sham operation group,model group,experimental-L,experimentalM,experimental-H groups were (160 ±24),(342 ±34),(324 ±29),(289 ±33),(253 ±37)pg · mL-1;(175±12),(120±14),(132 ±6),(145 ± 11),(155 ±15) pg · mL-1.Compared with sham operation group,IL-10 in model group were significantly lower and IL-6 was significantly higher(all P ＜ 0.05).Compared with model group,IL-6 in three doses experimental groups were significantly lower and IL-10 was significantly higher(all P ＜0.05).The lung injury score(point) in sham operation group,model group,experimental-M,experimental-H groups were 0.75 ±0.23,7.26 ± 0.52,3.65 ± 0.32,1.39 ± 0.25.The difference between sham operation group and model group while model group and experimental-M,-H groups were significantly (all P ＜ 0.05).Conclusion Pretreatment with Sheumai injection can reduce the lung injury induced through reducing the release of inflammatory cytokines in the lung.