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目的：探讨肝门部胆管癌组织中血管内皮细胞生长因子（ＶＥＧＦ）和血管生成的相关性。方法：应用逆转录多聚酶链反应（ＲＴ－ＰＣＲ）和免疫组化技术对２６例肝门部胆管癌、癌周组织及１２例正常组织中ＶＥＧＦｍＲＮＡ和蛋白及微血管密度（ＭＶＤ）进行了检测。结果：２６例肝门部胆管癌组织中ＶＥＧＦｍＲＮＡ阳性表达率为７６．９％（２０／２６）；癌周组织阳性表达率为２８．９％（７／２６）；正常组织表达率为８．３％（１／１２），三者差异有显著性（Ｐ＜０．０１）。ＶＥＧＦｍＲＮＡ阳性表达与ＶＥＧＦ蛋白表达具有一致性；ＶＥＧＦｍＲＮＡ阳性者ＭＶＤ值显著高于阴性者（Ｐ＜０．０１）；ＶＥＧＦｍＲＮＡ表达和ＭＶＤ与肝门部胆管癌的分化程度、浸润转移密切相关（Ｐ＜０．０５）；而与发生部位、病理类型、肿瘤大小、临床分型无关（Ｐ＞０．０５）。结论：ＶＥＧＦ在肝门部胆管癌发生和浸润转移过程中发挥重要作用，肿瘤血管生成与肝门部胆管癌浸润转移密切相关。
Objective: To investigate the correlation between vascular endothelial growth factor (VEGF) and angiogenesis in hilar cholangiocarcinoma. METHODS: VEGF mRNA and protein and microvessel density (MVD) were detected in 26 cases of hilar cholangiocarcinoma, pericancerous tissue, and 12 normal tissues using reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. Results: The positive expression rate of VEGF mRNA in hilar cholangiocarcinoma tissues was 76.9% (20/26). The positive expression rate of tissue peritumoral tissues was 28.9% (7/26). The positive tissue expression rate was 8. 3% (1/12), the difference was significant (P<0.01). The positive expression of VEGFmRNA was consistent with the expression of VEGF protein. The MVD value of VEGFmRNA positive group was significantly higher than that of negative(P<0.01). The expression of VEGFmRNA and MVD were closely related to the differentiation and invasion and metastasis of hilar cholangiocarcinoma (P< 0.05); and with the site of occurrence, pathological type, tumor size, clinical classification has nothing to do (P> 0.05). Conclusion: VEGF plays an important role in the occurrence, invasion and metastasis of hilar cholangiocarcinoma. Tumor angiogenesis is closely related to the invasion and metastasis of hilar cholangiocarcinoma.