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为探讨UFGT基因在观赏海棠叶片呈色过程中的作用,以观赏海棠常紫红色类品种‘王族’叶片总RNA为模板,通过RACE扩增,获得一个长度为2 186 bp的cDNA序列,其编码区共1 425 bp,编码475个氨基酸,命名为McUFGT。利用高效液相色谱法和实时荧光定量PCR技术,对3个不同叶色的观赏海棠品种‘王族’(叶片常紫红色类)、‘绚丽’(新叶红色类)和‘火焰’(叶片常绿色类)叶片中的花色素苷含量、McUFGT相对表达量进行测定分析。结果表明,在3个品种中矢车菊色素苷是主要的花色素苷物质,并且随着叶片的生长发育,不同叶色品种间矢车菊色素苷差异显著,其中以叶片常紫红色品种‘王族’矢车菊色素苷积累最多。同时矢车菊色素苷含量的变化与McUFGT相对表达量变化趋势基本一致,说明McUFGT在苹果属观赏海棠叶片花色素苷合成及色泽形成过程中具有重要的作用。
In order to investigate the role of UFGT gene in ornamental Begonia leaf color, a cDNA sequence of 2 186 bp in length was amplified by RACE using the total RNA of ornamental crabapple (Prunus armeniacae) A total of 1 425 bp, encoding 475 amino acids, named McUFGT. Using high performance liquid chromatography (HPLC) and real-time fluorescence quantitative PCR (RQ-PCR), the effects of three species of ornamental crabapple on the royal family (leaf reddish purple), ’brilliant’ (new leaf red) and ’flame’ Green type) leaf anthocyanin content, McUFGT relative expression was measured and analyzed. The results showed that cyanidin was the major anthocyanin in three cultivars, and with the growth and development of leaves, there were significant differences in cyanidin among different leaf color cultivars. Among them, Centauren has the highest accumulation of cyanidin. At the same time, the change of anthocyanin content in cyanidin was consistent with that of McUFGT, which indicated that McUFGT played an important role in anthocyanin synthesis and color formation in apple tree.