论文部分内容阅读
本研究以四季桂花瓣和叶为材料,采用RT-PCR和RACE技术获得四季桂DXPS基因c DNA全长,并用凝胶定量软件Quanlity-One对四季桂不同季节花瓣和叶片及不同花期花瓣的半定量PCR产物的凝胶图像进行分析。结果表明,四季桂DXPS基因c DNA全长2 185 bp(Gen Bank登录号:KT099324),其中阅读框长1 926 bp,编码641个氨基酸,含有保守功能结构域TPP-DXS、TPP-PYR-DXS-TK-like及Transketolase-C;半定量PCR结果分析表明,四季桂DXPS基因表达量在花朵盛开期最高,花蕾期次之,衰弱期最低;在花瓣中的表达量要明显高于同时期在叶片中的表达量;在花瓣中,12月份DXPS基因的相对表达量最高,3月份次之,在6月份和9月份DXPS相对表达量较低。研究结果初步阐明了DXPS基因在四季桂开花过程中的表达模式,可为深入研究香气合成途径及开展花香植物的分子育种提供科学基础。
In this study, Osmanthus fragrans petals and leaves were used as materials. The full-length c DNA of DXQ gene from Siji Gui was obtained by RT-PCR and RACE. The semi-quantitative The gel images of the quantitative PCR products were analyzed. The results showed that the full-length cDNA of DXQ gene from Siji Gui was 2 185 bp (Gen Bank accession number: KT099324), which contained 1 926 bp of reading frame and encoded a 641 amino acid sequence with conserved functional domains TPP-DXS and TPP-PYR-DXS -TK-like and Transketolase-C. The results of semi-quantitative PCR showed that the expression of DXQ gene in Siji Gui was the highest in the blossoming stage, followed by the budding stage and the weakest stage. The expression level of DXPS in the petals was significantly higher than that in the same period The expression of DXPS gene in petal was the highest in December, followed by March, and the relative expression of DXPS was lower in June and September. The results preliminarily clarified the expression pattern of DXPS in the flowering process of Siji Gui, which could provide a scientific basis for further study of aroma synthesis pathway and molecular breeding of floral plants.