论文部分内容阅读
本研究以‘富士’苹果红色芽变品种‘烟富8’果皮为试验材料,采用RT-PCR方法,克隆获得苹果UV-B受体基因UVR8的全长序列,命名为Md UVR8。结果显示,开放阅读框长度为1 359 bp,编码452个氨基酸,相对分子质量48.487 k D,等电点(PI)为5.56。蛋白质保守域分析表明,苹果UVR8蛋白包含7个RCC1结构域。蛋白质二级结构预测显示,苹果UVR8蛋白含有10个琢-螺旋,16个茁折叠,41个茁-转角。氨基酸同源性比对分析表明,苹果UVR8与已报道的其他植物的氨基酸序列相似性在69.54%~88.94%之间。核苷酸聚类分析表明,苹果和白梨首先聚为一类,其次是梅。本研究为苹果光受体对光应答的分子机理的进一步研究奠定了基础。
In this study, the full-length sequence of the UVRB gene of apple UV-B receptor was cloned by RT-PCR using ’Fuji’ apple red bud variety’Yunfu 8’ peel as Md UVR8. The results showed that the open reading frame was 1 359 bp in length, encoding 452 amino acids with a relative molecular mass of 48.487 kD and an isoelectric point (PI) of 5.56. Protein conserved domain analysis showed that apple UVR8 protein contains seven RCC1 domains. Protein secondary structure prediction shows that the apple UVR8 protein contains 10 cut - spiral, 16 Zhuo folding, 41 Zhuo - corner. Amino acid homology comparison analysis showed that the amino acid sequence similarity between apple UVR8 and other reported plants ranged from 69.54% to 88.94%. Nucleotide cluster analysis showed that apple and white pear were clustered first, followed by plum. This study laid the foundation for the further study of the molecular mechanism of photoresection in apple photoreceptors.