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目的：为了研究ＤＣＣ基因的杂合性丢失（ＬＯＨ）和Ｂｃｌ－２蛋白过度表达在胃癌发生中的作用，方法：采用ＰＣＲ方法及免疫组织化学技术检测了胃癌组织ＤＣＣ基因的ＬＯＨ和Ｂｃｌ－２蛋白的表达。结果：结果发现，胃癌（信息个体）ＬＯＨ发生率为５４５％（１８／３３），Ｂｃｌ－２蛋白表达阳性率为６０％（３０／５０）；ⅢⅣ期胃癌ＬＯＨ发生率（８２２％，１５／１８）显著高于ⅠⅡ期胃癌２０％，３／１５）（Ｐ＜００１）；Ｂｃｌ－２蛋白的表达与ＤＣＣ基因的ＬＯＨ及胃癌大小、分化、淋巴结转移、浆膜浸润、临床分期及Ｌａｔｌｒｅｎ’ｓ分型无显著相关。结论：以上结果提示，ＤＣＣ基因的ＬＯＨ和Ｂｃｌ－２蛋白的高表达均参与了胃癌的发生，ＤＣＣ基因的ＬＯＨ是胃癌发生的晚期事件，其致癌机制与Ｂｃｌ－２有所不同
OBJECTIVE: To study the role of loss of heterozygosity of DCC gene (LOH) and overexpression of Bcl-2 protein in gastric carcinogenesis. Methods: The LOH and Bcl-2 of DCC gene in gastric cancer tissues were detected by PCR and immunohistochemistry. Protein expression. Results: The results showed that the incidence of LOH in patients with gastric cancer (individuals of information) was 54.5% (18/33), and the positive rate of Bcl-2 protein expression was 60% (30/50); the incidence of LOH in stage III-IV gastric cancer (82 2%, 15/18) was significantly higher than that of stage I-II gastric cancer 20%, 3/15) (P<001); Bcl-2 protein expression was correlated with DCC gene LOH and gastric cancer size, differentiation, lymph node metastasis There was no significant correlation between plasma membrane infiltration, clinical stage, and Latlren’s classification. Conclusion: The above results suggest that the high expression of LOH and Bcl-2 protein in DCC gene is involved in the occurrence of gastric cancer. The LOH of DCC gene is a late event in gastric cancer. The mechanism of carcinogenesis is different from that of Bcl-2.