论文部分内容阅读
目的 :探讨γ射线和高热单独及联合暴露对胚胎脑发育的影响 方法 :对孕 9d小鼠给予 1 0Gy60 Co -γ射线照射或 /和 42℃ 10min高热处理 ,孕 18d剖腹取胎 ,观察胎鼠外观 ,测定胎鼠脑重、全脑细胞数、脑组织核酸及蛋白质含量和作为胆碱能神经元标志的脑组织乙酰胆碱脂酶 (AChE)活力。结果 :各组胎鼠均无神经管畸形发生 ;与对照组比较 ,单纯照射组各指标均显著降低 ,单纯高热组全脑细胞数和AChE活力显著降低 ;先高热处理 ,4h后再照射 ,脑组织AChE活力显著高于单纯照射组 ;先照射 ,后高热处理 ,各指标与单纯照射组无显著差异。结论 :42℃ 10min高热处理对鼠胚胎脑发育的影响远小于 1 0Gyγ照射 ;预先给予高热处理可诱导胚胎对随后给予的γ辐射产生交叉适应性反应 ;先照射 ,后高热处理 ,两因素未呈现明显相加或协同作用
Objective: To investigate the effects of γ-rays and hyperthermia alone and in combination on embryonic brain development.Methods: Pregnant 9d mice were treated with 10Gy60Co-γ ray or / and hyperthermia at 42 ℃ for 10min, Appearance, determination of fetal rat brain weight, total brain cells, brain tissue nucleic acid and protein content and acetylcholinesterase (AChE) activity as a marker of cholinergic neurons. Results: Compared with the control group, all the indexes in the irradiation group were significantly lower than those in the control group, and the number of brain cells and AChE activity in the hyperthermia group were significantly decreased; The activity of AChE in the tissue was significantly higher than that in the pure irradiation group. The first irradiation and the later high heat treatment showed no significant difference between each index and the simple irradiation group. Conclusion: The effect of heat treatment at 42 ℃ for 10 min on the brain development of mouse embryos is much less than that of 10 Gy irradiation. Preimplantation heat treatment can induce the embryos to cross-react with the gamma radiation subsequently. The first exposure and the second post-heat treatment are not presented Obvious additive or synergistic effect