UPLC法同时测定重连口服液中5种成分的含量

• 作 者: 韩燕全 李泽庚 洪燕 夏伦祝 高家荣 汪永忠 李雪苓
• 机 构: 安徽中医药大学第一附属医院/国家中医药管理局中药制剂三级实验室,安徽中医药大学,
• 出 处: 中药材
• 发表日期: 2013年12期
• 关键词: 口服液 UPLC 重连口服液 射干苷 黄芩苷 连翘苷 磷酸溶液 梯度洗脱 阴性对照品 峰面积

目的:采用UPLC法同时测定重连口服液中5种有效成分(射干苷、黄芩苷、连翘苷、黄芩素和汉黄芩素)的含量。方法:色谱柱为Waters HSS T3 C18柱(2.1 mm×50 mm,1.8μm);流动相为乙腈-0.05%磷酸溶液,梯度洗脱;流速为0.25 mL/min;柱温为30℃;检测波长为270 nm。结果:5种成分浓度与峰面积的线性关系良好(r>0.9998),加样回收率为97.90%~100.45%。结论:该方法简便、快捷、重复性好,可用于重连口服液的质量控制。

OBJECTIVE: To simultaneously determine the contents of five active ingredients (cantharidin, baicalin, forsythin, baicalein and wogonin) in Chongqing Lianlian Oral Liquid by UPLC. METHODS: The column was a Waters HSS T3 C18 column (2.1 mm × 50 mm, 1.8 μm). The mobile phase consisted of acetonitrile-0.05% phosphoric acid solution with gradient elution. The flow rate was 0.25 mL / min and the column temperature was 30 ℃. The detection wavelength Is 270 nm. Results: The linear relationship between the concentration of five components and the peak area was good (r> 0.9998). The average recoveries were 97.90% -100.45%. Conclusion: The method is simple, rapid, reproducible and can be used for the quality control of rehydration oral liquid.