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对Botryosphaeria dothidea通过皮孔侵入果实的过程进行了光学显微镜和扫描电镜观察,发现接种后2d分生孢子完成萌发和附着胞形成过程。接种后10d,菌丝体在皮孔表面缓慢生长。从20￣30d菌丝体加速扩展并形成几个分枝,通常从皮孔外围侵入果实,但仅限于皮孔表面组织。从40～50d,菌丝体扩展更为繁茂,一些菌丝开始突入皮孔的第2层组织。接种皮孔中可检测到多聚半乳糖醛酸酶(PG),但活性比对照高。皮孔有3层,第1层为含果胶的死组织,易被病菌侵染降解,底部的第3层非常坚固,是阻止病菌侵染的有效屏障。随果实发育,皮孔大小、数目和裂口均有规律地增加。我们的研究结果进一步证实了苹果轮纹病的潜伏侵染特性。
Botryosphaeria dothidea penetrated into the fruit through the lenticel process by light microscopy and scanning electron microscopy, found 2d after inoculation conidia germination and adherence formation process. Ten days after inoculation, mycelia grew slowly on the surface of lenticels. The mycelium spreads rapidly and forms several branches from 20 to 30 days, usually penetrating fruit from the periphery of the lenticel, but only to the lenticel surface tissue. From 40 to 50 d, the mycelium expanded more lush, and some mycelia began to penetrate into the second layer of the lenticel. Polygalacturonase (PG) was detected in the inoculated skin, but had higher activity than the control. There are three layers of lenticels, the first layer of dead tissue containing pectin, susceptible to pathogen degradation, the bottom of the third layer is very solid, is to prevent the infection of bacteria effective barrier. With the development of fruit, the size of lenticels, the number and the nicks increased regularly. Our results further confirm the latent infection characteristics of apple ring disease.