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目的：证实兔视网膜中有α、β和γ三种蛋白激酶Ｃ（ｐｒｏｔｅｉｎｋｉｎａｓｅＣ，ＰＫＣ）亚型的分布。方法：采用免疫组织化学技术，以抗ＰＫＣ同功酶Ⅰ（α）、Ⅱ（β）、Ⅲ（γ）单克隆抗体对兔视网膜进行ＰＫＣ同功酶定位研究。结果：兔视网膜中有ＰＫＣ－α、ＰＫＣ－β和ＰＫＣ－γ的阳性免疫反应。ＰＫＣ－α免疫反应主要呈现在内核层的双极细胞；ＰＫＣ－β免疫反应主要呈现在神经节细胞层的神经节细胞；ＰＫＣ－γ则在神经节细胞层、内网状层以及光感受器的外节呈弥漫性弱阳性染色。结论：作为中枢神经系统的一部分，视网膜可以同时存在ＰＫＣ－γ、ＰＫＣ－β和ＰＫＣ－α三种亚型。
Objective: To confirm the distribution of three protein kinase C (PKC) subtypes α, β and γ in rabbit retina. Methods: Immunohistochemical technique was used to study the PKC isoenzyme localization of rabbit retina with anti-PKC isozymes Ⅰ (α), Ⅱ (β) and Ⅲ (γ) monoclonal antibodies. Results: The rabbit retina with PKC-α, PKC-β and PKC-γ positive immune response. PKC-α immunoreactivity mainly appears in the inner nuclear layer of bipolar cells; PKC-β immunoreactivity mainly in the ganglion cell ganglion cells; PKC-γ in the ganglion cell layer, the reticular layer and the photoreceptor External section was diffuse weakly positive staining. Conclusion: As part of the central nervous system, the retina can co-exist PKC-γ, PKC-β and PKC-α three subtypes.