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用放射配体分析法，研究长时间失血性休克大鼠肾脏组织糖皮质激素受体（ＧＲ）变化并检测磷酯酶Ａ２（ＰＬＡ２）变化。结果显示：休克２小时后，ＧＲ的最大结合容量（Ｒ０）开始下降，并随休克时间延长而继续下降，于８小时后，ＧＲ的Ｒ０仅为对照组的２１％。对糖皮质激素（ＧＣ）的亲和力也下降。同时肾脏ＰＬＡ２上升，与ＧＲ的Ｒ０下降呈负相关（ｒ＝０．７１．Ｐ＜０．０５）。说明ＧＲ功能下降所致受体水平ＧＣ功能不全对长时间失血性休克的发生、发展及与休克后肾功能损害可能有关。
Radioligand analysis was used to study the changes of glucocorticoid receptor (GR) in kidney and the changes of phospholipase A2 (PLA2) in rats after long-term hemorrhagic shock. The results showed that after 2 hours of shock, the maximum binding capacity (R0) of GR began to decrease and continued to decrease with the prolongation of shock time. After 8 hours, R0 of GR was only 21% of the control group. The affinity for glucocorticoids (GC) also decreased. At the same time, PLA2 increased in kidney, which was negatively correlated with the decrease of R0 in GR (r = 0.71, P <0.05). These results suggest that the GC level of receptor dysfunction induced by the decline of GR may be related to the occurrence and development of long-term hemorrhagic shock and the impairment of renal function after shock.