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利用顶空固相微萃取-气相色谱质谱技术(HS-SPME/GC-MS),建立了一种同时检测啤酒中基于不同老化反应的11种老化指示性物质的定量分析方法。通过优化萃取头种类、萃取时间、萃取温度等条件,最终确定HS-SPME最佳萃取条件为使用2cm的DVB/CAR/PDMS(涂层厚50/30μm)萃取头、50℃下萃取40 min。质谱采集SIM模式,内标物质使用对氟苯甲醛。该方法的线性良好(R2>0.99),准确性高(11种物质的加标回收率在82.6%～102%之间),稳定性强(相对标准偏差均低于16%),能同时准确检测出麦汁、发酵液和啤酒中反映不同老化机理的不同老化指示物质的含量,适用于啤酒老化研究和调控的日常检测。
A quantitative method for the simultaneous determination of 11 indicators of aging based on different aging reactions in beer was established by headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME / GC-MS) The optimum extraction conditions of HS-SPME were determined by optimizing the conditions of extraction head, extraction time and extraction temperature. The extraction conditions were as follows: 2 cm DVB / CAR / PDMS (50/30 coating thickness) extraction head and 40 min extraction at 50 ℃. Mass acquisition SIM mode, the internal standard substance using fluorobenzaldehyde. The method has good linearity (R2> 0.99), high accuracy (the recoveries of 11 substances are between 82.6% and 102%) and the stability is good (the relative standard deviations are less than 16%), which can be accurate Detection of wort, fermentation broth and beer reflect different aging mechanisms of different aging indicator substance content, suitable for beer aging research and regulation of routine testing.