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采用毛细管电泳,以10 mmol/L柠檬酸+14 mmol/L三羟甲基氨基甲烷+45 mmol/L二甲基-β-环糊精为电泳运行液,在熔融石英毛细管(50μm id×45 cm,有效长度40 cm)中,正高压分离(+16 kV),进样时间为10 s,手性药物羟苄唑获得了良好的基线分离,线性范围为5.0~120 mg/L,检出限为1.5mg/L。羟苄唑对映体的分离度为2.25。考察了电泳运行液组成、手性选择剂的浓度、有机改性剂种类、进样方式等对灵敏度和分离度的影响,为羟苄唑对映体的分离检测提供了可行的分析方法。
Using capillary electrophoresis, 10 mmol / L citric acid + 14 mmol / L trimethylamine + 45 mmol / L dimethyl-β-cyclodextrin as electrophoresis running solution, fused silica capillary (50μm id × 45 cm and an effective length of 40 cm), positive high pressure separation (+16 kV) and injection time of 10 s, the baseline separation of chiral drugs was achieved with a linear range of 5.0 ~ 120 mg / L Limit to 1.5mg / L. The resolution of the benzoxazole enantiomer was 2.25. The effects of composition of electrophoresis running liquid, concentration of chiral selector, type of organic modifier and injection mode on sensitivity and resolution were investigated. This provided a feasible analytical method for the separation and detection of enantiomers of benzyl.