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采用超声提取法提取不同产地核桃不同部位总黄酮,以木犀草苷为对照品,采用HPLC法和比色法测定提取物中木犀草苷和总黄酮含量,比较不同产地核桃隔膜、种皮及壳中木犀草苷及总黄酮的含量差异,为药用核桃的道地性及药用部位提供依据。结果表明:不同产地核桃中木犀草苷含量为长治>和田>阿克苏>塔里木>大理>秦岭,总黄酮含量为大理>秦岭>和田>塔里木>长治>阿克苏。核桃不同组织部位中木犀草苷的分布为种皮>壳>隔膜,不同组织部位中总黄酮的分布为壳>种皮>隔膜。表明,不同产地核桃的黄酮成分含量有差别,山西核桃木犀草苷含量最高,但总黄酮含量最低;云南核桃总黄酮含量最高,但木犀草苷含量很低。核桃种皮中木犀草苷及总黄酮含量要高于隔膜中的,核桃种皮可能和隔膜有相似的药理活性,同样具有很好的药用价值。
Ultrasonic extraction was used to extract total flavonoids in different parts of walnut from different areas. The contents of luteolin and total flavonoids in extracts were determined by HPLC and colorimetric method. The contents of flavonoids, In the luteolin and total flavonoids content differences for medicinal walnut authenticity and medicinal sites provide the basis. The results showed that the contents of luteolin in walnut from different areas were as follows: Changzhi> Hetian> Aksu> Tarim> Dali> Qinling. The content of total flavonoids was Dali> Qinling> Wada> Tarim> Changzhi> Aksu. The distribution of luteolin in different tissues of walnut was as seed coat> shell> septum. The distribution of total flavonoids in different tissues was shell> seed coat> septum. The results showed that the contents of flavonoids in walnut from different habitats were different. The content of luteolin in Shanxi walnut was the highest but the content of total flavonoids was the lowest. The content of flavonoids in Yunnan walnut was the highest, but the content of luteolin was low. The content of luteolin and total flavonoids in the walnut seed coat is higher than that in the septum. The walnut seed coat may have similar pharmacological activities with the septum, and also has good medicinal value.