论文部分内容阅读
为探讨人鼻咽癌染色体端粒行为异常与癌变的关系，应用 Ｓｏｕｔｈｅｒｎ 印迹杂交对 ３０ 例鼻咽癌和３０例与之年龄、性别配对的正常对照进行了分析。结果：鼻咽癌的端粒长度与对照相比显著缩短。在鼻咽癌中，１６１７％ 显示端粒伸长，７６．６７％ 显示端粒缩短，而６．６７％ 显示与对照相同的端粒长度。分析了 ３０ 例正常人外周血淋巴细胞端粒长度的变化，可见染色体端粒的长度随年龄增长而逐渐缩短（ｒ＝ － ０．３９１３， Ｐ＜ ０．０５），并且端粒每年平均缩短 ３９ｂｐ 的核苷酸。结果提示：人鼻咽癌染色体不稳定可能与端粒长度缩短有关。
In order to investigate the relationship between telomere abnormality and carcinogenesis of human nasopharyngeal carcinoma, Southern blot hybridization was used to analyze 30 cases of nasopharyngeal carcinoma and 30 normal controls matched with their age and sex. Results: Telomere length of NPC was significantly shorter than that of the control. In NPC, 16.17% showed telomere elongation, 76.67% showed telomere shortening, and 6.67% showed the same telomere length as the control. The changes of telomere length of peripheral blood lymphocytes in 30 normal subjects were analyzed. The telomere length of chromosomes was gradually shortened with age (r = - 0.3913, P <0.05), and the telomere shortened by 39 bp Of nucleotides. The results suggest that the chromosomal instability of human nasopharyngeal carcinoma may be related to the shortened telomere length.