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用电生理及免疫细胞化学方法观察急性脑缺血大鼠海马及运动皮质的脑电图和其内ＧＡＢＡ能神经元的变化。结果表明：夹闭双侧颈总动脉１５ｍｉｎ后，海马及运动皮质的脑电波波幅变低、频率变慢、高幅θ波增多，有时呈短程阵发性出现。这些变化随着缺血的发展而更加明显，有时出现癫痫样棘波或棘慢波或阵发性尖波。缺血１ｈ后，脑电波有所恢复，但没有恢复到正常水平。免疫细胞化学染色结果：缺血３０ｍｉｎ后，海马及运动皮质内ＧＡＢＡ能神经元开始减少，２ｈ后明显减少。此时海马内约为对照组的６０％左右，表现最明显的部位为齿状回和ＣＡ１及ＣＡ３区。运动皮质内比对照组减少５０％左右。结果提示：急性弥散性不完全性脑缺血时，由于脑血供减少，导致抑制性ＧＡＢＡ能神经元数量减少，出现海马及运动皮质脑电图和行为明显改变，这些变化可能与脑缺血所致的机能障碍和临床表现有关。
Electrophysiological and immunocytochemical methods were used to observe the changes of EEG and GABAergic neurons in hippocampus and motor cortex of rats with acute cerebral ischemia. The results showed that: After clipping bilateral common carotid arteries for 15 minutes, the amplitude of EEG in the hippocampus and motor cortex became lower and the frequency became slower. The amplitude of high amplitude θ wave increased, sometimes in short-range paroxysmal. These changes are more pronounced with the development of ischemia, with epileptiform spikes or spikes or paroxysmal spikes. 1h after ischemia, brain waves recovered, but did not return to normal levels. Immunocytochemical staining results: GABAergic neurons in the hippocampus and motor cortex began to decrease after 30min ischemia, but decreased significantly after 2h. At this time, the hippocampus was about 60% of the control group, with the most obvious sites being the dentate gyrus and the CA1 and CA3 areas. Motor cortex less than the control group about 50%. The results suggest that in acute diffuse incomplete cerebral ischemia, the decrease of cerebral blood supply leads to the decrease of the number of inhibitory GABAergic neurons, the change of EEG and behavior of hippocampus and motor cortex, which may be associated with cerebral ischemia The resulting dysfunction and clinical manifestations.