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利用凝胶切割纯化法得到免疫纯人血清白蛋白(ALB),经聚丙烯酰胺凝胶(PAC)电泳确认为单一带,经SDS-PACE确定其相对分子质量为67462.80U,与Sigma纯品ALB及日本产羊抗人ALB抗血清免疫双扩散融合试验验证其为纯品ALB。利用得到的ALB免疫绵羊制备羊抗人白蛋白抗血清,与日本产品比较,为单价特异性抗血清。
Immuno-pure human serum albumin (ALB) was obtained by gel-cutting and purification method. The polyacrylamide gel (PAC) electrophoresis was used as a single band. The relative molecular mass was 67462.80U by SDS-PACE, ALB and Japanese goat anti-human ALB antiserum double immunodiffusion fusion test proved to be pure ALB. The obtained sheep immunized with ALB were used to prepare goat anti-human albumin antiserum, which was monovalent specific antiserum compared with Japanese products.