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本文研究了致癌物苯并(a)芘(Bap)的同步荧光光谱测定法,通过选取一定的激发和发射单色器的波长差(△λ)来提高选择性,并用峰高进行定量计算。将此法测定实际样品的结果与环境监测中迄今所用的窄基线法进行了比较,二者一致性很好,且前者更简便,可靠。基本原理在常规的荧光法中,通常是根据所获得的激发光谱和发射光谱进行定性定量分析的。1971年Lloyd首先提出了同步光谱法,在激发和发射单色器间固定一定的波长差(△λ)而
In this paper, the simultaneous fluorescence spectrometry of carcinogenic benzo (a) pyrene (Bap) was studied. The selectivity was improved by selecting the wavelength difference (△ λ) of the excitation and emission monochromator and the peak height was used for quantitative calculation. The results of this method for the determination of actual samples were compared with the narrow baseline method used so far in environmental monitoring. The agreement between the two is very good and the former is simpler and more reliable. Rationale In conventional fluorescence methods, qualitative and quantitative analysis is usually based on the excitation and emission spectra obtained. In 1971, Lloyd first proposed synchronous spectroscopy, which fixed a certain wavelength difference (Δλ) between the excitation and emission monochromators