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目的 以 ｍ Ｒ Ｎ Ａ 差异显示技术对比研究具有不同转移潜能的癌系，克隆与肿瘤转移相关的基因。方法 以肺巨细胞癌细胞系 Ｐ Ｇ 具有不同转移潜能的亚系（ Ｂ Ｅ１， Ｌ Ｈ７）为研究对象，应用 ｍ Ｒ Ｎ Ａ 差异显示技术克隆差异片段，经 Ｎｏｒｔｈｅｒｎ 杂交验证后测序，进入 Ｇｅｎ Ｂａｎｋ 数据库检验同源性。结果 ｍ Ｒ Ｎ Ａ 差异显示技术对比高低转移癌系ｍ Ｒ Ｎ Ａ，分别建立了高低转移相关基因资源库。其中一个８４９ ｂｐ 片段在高转移亚系（ Ｂ Ｅ１）低表达，而在不转移亚系（ Ｌ Ｈ７）高表达，两者差异显著，经 Ｇｅｎ Ｂａｎｋ Ｂｌａｓｔｎ 检索，与 Ｇ３ Ｂ Ｐ（ Ｒａｓ Ｇ Ｔ Ｐａｓｅ Ａｃｔｉｖａｔｉｎｇ ｐｒｏｔｅｉｎ Ｓ Ｈ３ ｄｏｍ ａｉｎ ｂｉｎｄｉｎｇｐｒｏｔｅｉｎ）同源性高达 ９９％ 。结论 在高低转移癌系中 Ｇ３ Ｂ Ｐ的表达具有显著差异，提示 Ｇ３ Ｂ Ｐ与肿瘤的转移表型有一定相关性，为 Ｇ３ Ｂ Ｐ功能的研究提供了新的线索。
Objective To compare and study the cancer lines with different metastatic potential by m R N A differential display technology and clone the genes related to tumor metastasis. METHODS: The subclones (B E1, L H7) with different metastatic potentials of the giant cell lung cancer cell line P G were used as research objects. The differential fragments were cloned using the m R N A differential display technique and verified by Northern hybridization and sequenced to enter the Gen Bank. The database checks for homology. Results The m R N A differential display technique contrasted m R N A with high- and low-metastatic cancer lines, and established high and low metastasis-related gene pools. One of the 849 bp fragments was lowly expressed in the high metastatic subgroup (B E1) and highly expressed in the non-metastatic subgroup (L H7). There was a significant difference between the two, with Gen Bank Blastn searching and G3 B P (RasG T). Pase-Activating protein S H3 dom ain binding protein) has a homology of up to 99%. Conclusion There is a significant difference in the expression of G3 B P in high and low metastatic cancer lines, suggesting that G3 B P has a certain correlation with the metastasis phenotype of tumors, providing new clues for the study of G3 B P function.