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应用淋巴细胞杂交瘤技术，建立了４株抗人血清肿瘤碱性蛋白（Ｔｕｍｏｕｒｂａｓｉｃｐｒｏ－ｔｅｉｎ，ＴＢＰ）杂交瘤细胞株（１Ｃ３、４Ｆ４、５Ｆ４、３Ｇ９），并以制备的单克隆抗体（ＭｃＡｂ）对其抗原决定簇及免疫学特性进行了分析。Ｉｇ亚类测定：均为ＩｇＧ２ａ，腹水效价为１×１０－６～１×１０－８。特异性测定：ＴＢＰＭｃＡｂ与ＩｇＧ、ＩｇＡ、ＩｇＭ和Ａｌｂ无交叉反应。单抗相加试验证实：５Ｆ４、４Ｆ４和１Ｃ３为识别ＴＢＰ上同一抗原决定簇，３Ｇ９则为识别ＴＢＰ上另一抗原决定簇。分别利用单株和混合株ＭｃＡｂ标酶建立了可应用于人血清ＴＢＰ含量测定的ＥＬＩＳＡ双抗体夹心法，并用于人血清ＴＢＰ含量测定。
Four hybridoma cell lines (1C3, 4F4, 5F4 and 3G9) against Tumor of Serum Tumor were established by using lymphocyte hybridoma technique. The monoclonal antibodies (McAb) Its antigenic determinants and immunological properties were analyzed. Ig subclass determination: both IgG2a, ascites titer of 1 × 10-6 ~ 1 × 10-8. Specificity Assay: TBPMcAb did not cross-react with IgG, IgA, IgM and Alb. MAb addition assay confirmed: 5F4, 4F4 and 1C3 to identify the same epitope on TBP, 3G9 is to identify another epitope on TBP. The McAbs of single and mixed strains were used to establish the ELISA double antibody sandwich method which can be applied to the determination of human serum TBP content and used for the determination of human serum TBP content.