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材料和方法基本材料和方法同上文研究(一)。不同之处在各实验栏中注明。一、不同的甘油浓度对冻贮复温后的精子存活率的影响实验用10份精液标本。每份精液液化后均分为5份,按精液:保护剂=1:1之比,分别添加五种不同的保护剂,细管法分装,冷平衡30分钟,-110℃初冻温度冷冻,保护剂按甘油浓度不同分 A、B、C、D、E 五组,与精液混合后甘油最终浓度依次为3%、
Materials and methods The basic materials and methods with the above study (a). Differences in the experimental column indicate. First, the effect of different glycerol concentrations on the survival rate of sperm after frozen storage rewarming Experiment with 10 semen samples. Each semen liquefaction is divided into 5 parts, according to the ratio of semen: protective agent = 1: 1, respectively, add five different protective agent, thin tube method sub-assembly, cold balance for 30 minutes, -110 ℃ freezing temperature freezing , The protective agent according to the concentration of glycerol different points A, B, C, D, E five groups, and the final concentration of glycerol mixed with semen followed by 3%