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为探讨青春期饮酒致学习记忆力下降的可能机制,本研究观察了青春期大鼠酒精处理后海马CA1区脑源性神经营养因子(BDNF)和酪氨酸激酶受体B(TrkB)的表达变化。实验选用30d龄SD雄性大鼠,以25%的酒精按8g/kg/d灌胃,连续灌7d,动物分别在停酒后0d,3d,7d和14d处死;对照组以等量生理盐水代替酒精按同样方法处理。用免疫组织化学方法(ABC法)检测海马CA1区BDNF和TrkB的表达,Motic3.2图像分析系统测定免疫阳性产物的平均灰度值。结果显示,BDNF的表达在停酒后0d和14d,实验组与相应对照组相比差异无显著性(P>0.05);3d显著升高(P<0.05);7d明显下降(P<0.05)。TrkB的表达在停酒后0d,3d,7d,实验组与相应对照组相比差异无显著性(P>0.05);14d显著下降(P<0.05)。以上结果提示,BDNF表达的相对不足可能是青春期饮酒致学习记忆力持续性下降的原因之一。
In order to explore the possible mechanism of decreased learning and memory during adolescent drinking, the present study investigated the changes of brain-derived neurotrophic factor (BDNF) and tyrosine kinase receptor B (TrkB) in hippocampal CA1 after alcohol treatment in adolescent rats. The rats were sacrificed on the 0d, the 3d, the 7d and the 14d after the stop of the alcoholic beverages. The rats in the control group were given the same amount of normal saline instead Alcohol treatment by the same method. The expression of BDNF and TrkB in CA1 region of hippocampus was detected by immunohistochemical method (ABC method), and the average gray value of immunopositive products was determined by Motic3.2 image analysis system. The results showed that the expression of BDNF at 0d and 14d after alcohol withdrawal showed no significant difference between the experimental group and the corresponding control group (P> 0.05), increased significantly on 3d (P <0.05), significantly decreased on the 7th day (P <0.05) . There was no significant difference in the expression of TrkB between the experimental group and the corresponding control group on the 0d, 3d, 7d after alcohol withdrawal (P> 0.05), and significantly decreased on the 14th day (P <0.05). The above results suggest that the relative lack of BDNF expression may be one of the reasons for the sustained decline in learning and memory caused by alcohol consumption during adolescence.