论文部分内容阅读
通过克隆脑膜炎奈瑟氏菌NhhA基因GNA0992于原核表达载体pET-20b,在大肠杆菌BL21(DE3)中实现了可溶性表达,表达量约占菌体蛋白总量的20%。经初步纯化后免疫小鼠,对其免疫原性进行了初步分析。结果显示,经3次腹腔免疫,血清IgG滴度达到23186,同时杀菌力实验显示NhhA能诱导针对B群脑膜炎奈瑟氏菌的补体依赖的杀菌反应。证明了NhhA是一种良好的抗原,为疫苗开发的蛋白靶标筛选工作奠定了基础。
The NhhA gene GNA0992 was cloned into the prokaryotic expression vector pET-20b to obtain the soluble expression in E. coli BL21 (DE3), which accounted for about 20% of the total bacterial proteins. The mice were immunized after preliminary purification, and their immunogenicity was analyzed preliminarily. The results showed that three times of intraperitoneal immunization, serum IgG titer reached 23,186, while bactericidal experiments showed that NhhA can induce a complement-dependent bactericidal response against N. meningitidis group B Neisseria gonorrhoeae. It is proved that NhhA is a good antigen, which lays the foundation for the screening of protein targets for vaccine development.