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目的了解碱性成纤维细胞生长因子(bFGF)对海马伞切断造成的阿尔茨海默病(AD)模型大鼠海马齿状回神经发生的影响,探讨bFGF治疗AD的前景。方法36只SD大鼠随机分成正常对照组(n=12)、AD对照组(n=12)和AD处理组(n=12)。AD处理组及AD对照组大鼠左侧海马伞切断制造AD模型;正常对照组注射生理盐水。AD处理组造模后每天侧脑室注射bFGF共7d;AD对照组及正常对照组同时间注射生理盐水。BrdU标记增殖细胞。TUNEL方法标记DNA片段,原位检测凋亡细胞。计数海马齿状回BrdU阳性细胞与凋亡细胞数。结果AD处理组大鼠与AD对照组大鼠相比,海马齿状回BrdU阳性细胞增加犤两组在颗粒细胞层阳性细胞分别为(163±37),(53±5)个/切片平面;海马门(28.5±5.5),(12.3±2.8)个/切片平面;分子层(10.7±2.2),(6.0±1.4)个/切片平面犦,差异有非常显著性意义(F=103.4,83.4,33.4,P<0.01),而凋亡细胞差异无显著性意义(P>0.05)。AD对照组大鼠与正常对照组大鼠相比,海马齿状回BrdU阳性细胞数及凋亡细胞差异均无显著性意义(P>0.05)。结论bFGF可刺激AD模型大鼠海马神经发生。
Objective To investigate the effect of basic fibroblast growth factor (bFGF) on the hippocampal dentate gyrus in Alzheimer’s disease (AD) model rats induced by transection of the hippocampus and to explore the prospect of bFGF in the treatment of AD. Methods Thirty - six SD rats were randomly divided into normal control group (n = 12), AD control group (n = 12) and AD treatment group (n = 12). The rats in AD group and AD control group were cut off from the left hippocampus to make AD model. Normal control group was injected with normal saline. The rats in AD group were given intracerebroventricular injection of bFGF daily for 7 days after modeling. The rats in AD group and normal control group were injected with saline at the same time. BrdU labeled proliferating cells. The TUNEL method was used to label DNA fragments and the apoptotic cells were detected in situ. The numbers of BrdU positive cells and apoptotic cells in hippocampal dentate gyrus were counted. Results The number of BrdU positive cells in hippocampal dentate gyrus in AD group was significantly higher than that in AD group (163 ± 37) and (53 ± 5) / slice in the granulosa cells respectively. (28.5 ± 5.5) and (12.3 ± 2.8) / slice planes in the hippocampus, and the molecular layer (10.7 ± 2.2) and (6.0 ± 1.4) / slice planes were significantly different (F = 103.4,83.4, 33.4, P <0.01), while the apoptotic cells had no significant difference (P> 0.05). There were no significant differences in BrdU positive cells and apoptotic cells between AD control group and normal control group (P> 0.05). Conclusion bFGF can stimulate hippocampal neurogenesis in AD model rats.