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目的探讨黄芪有效成分抗PC12细胞缺氧损伤的作用。方法超声醇水法提取黄芪有效成分,包括醇溶部分的黄酮、皂苷及水溶部分的黄芪多糖。用连二亚硫酸钠(Na2S2O4)诱导PC12细胞建立缺氧损伤模型,然后加入不同剂量的黄芪提取物,以四甲基偶氮唑盐(MTT)比色法及乳酸脱氢酶(LDH)漏出率测定法观察黄芪有效成分抗PC12细胞缺氧损伤作用。结果缺氧损伤可引起PC12细胞发生明显的损伤性变化,MTT测定的吸光度OD值下降,LDH漏出增加;在损伤后黄酮类可减少LDH漏出,增加吸光度OD值,使细胞存活率明显提高,以终浓度4mg/m l的作用最明显,在缺氧损伤后6h内抗损伤作用最佳,而多糖类此作用不明显。结论黄芪有效成分黄酮类有显著抗Na2S2O4诱导PC12细胞缺氧损伤作用。
Objective To investigate the effect of active ingredients of Radix Astragali on hypoxic injury of PC12 cells. Methods Ultrasonic alcohol-water method was used to extract the effective components of Radix Astragali, including flavonoids, saponins from the alcohol-soluble fractions and astragalus polysaccharides from the water-soluble fractions. An hypoxic injury model was established in PC12 cells induced by sodium hyposulfite (Na2S2O4). Different doses of Astragalus membranaceus extract were added to determine the leakage rate of MTT colorimetric assay and lactate dehydrogenase (LDH). The method was used to observe the anti-hypoxic effect of the active ingredients of Radix Astragali on PC12 cells. RESULTS: Hypoxic injury could cause significant damage changes in PC12 cells. The absorbance OD value measured by MTT decreased, and LDH leakage increased. After injury, flavonoids could reduce LDH leakage, increase the absorbance OD value, and significantly increase the cell survival rate. The effect of the final concentration of 4 mg/ml was the most obvious, and the anti-injury effect was best within 6 h after the anoxic injury, while the effect of the polysaccharide was not obvious. Conclusion Flavonoids from the effective components of Radix Astragali have a significant anti-anoxic effect on Na2S2O4-induced PC12 cells.