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BACKGROUND:The anti-proliferative gene,PC3(pheoch- romocytoma cell 3)/BTG2(B-cell translocation gene 2), is one of the early growth response genes and belongs to the BTG/Tob protein family.This study aimed to assess the effects of recombinant human hepatopoietin(HPO) and partial hepatectomy on rapidly induced expression of immediate-early genes and to investigate the expression of PC3/BTG2 mRNA in hepatocellular carcinoma(HCC)at different stages of progression. METHODS:After a rat model of partial hepatectomy was established,we investigated gene expression within 1 hour after 2/3 partial hepatectomy by representational difference analysis and in a primary cultured hepatocyte system.The expression levels of PC3/BTG2 from liver tissues of the rat model were assessed by RT-PCR and Northern blotting. Meanwhile,the expression of BTG2 mRNA in a tissue microarray of HCC was determined by in situ hybridization. RESULTS:The PC3/BTG2 gene was rapidly induced after 2/3 partial hepatectomy and its expression peaked within 1-2 hours after operation.HPO rapidly induced the expression of the genes c-fos,LRF-1,and PC3 in primary cultured rat hepatocytes,which might be one of the molecular mechanisms by which HPO stimulates hepatocyte proliferation.Positive BTG2 mRNA expression was detected in 71.19%(42/59)of the HCC samples and in 75%(3/4)of the normal liver tissue samples obtained from the region around the HCC tissues.PC3/BTG2 mRNA was located mainly in the cytoplasm of HCC cells and its expression was related to the degree of differentiation. CONCLUSIONS:Recombinant human HPO and partial hepatectomy rapidly induce the expression of the PC3/ BTG2 gene.PC3/BTG2 mRNA is highly expressed in HCC cells and its expression is related to the degree of cell differentiation.The abnormal expression of PC3/BTG2 is closely related to the genesis and development of HCC,so PC3/BTG2 may play an important role in these processes.
BACKGROUND: The anti-proliferative gene, PC3 (pheochromocytoma cell 3) / BTG2 (B-cell translocation gene 2), is one of the early growth response genes and belongs to the BTG / Tob protein family. This study aimed at assessing the effects of recombinant human hepatopoietin (HPO) and partial hepatectomy on rapidly induced expression of immediate-early genes and to investigate the expression of PC3 / BTG2 mRNA in hepatocellular carcinoma (HCC) at different stages of progression. METHODS: After a rat model of partial hepatectomy was established, we investigated gene expression within 1 hour after 2/3 partial hepatectomy by representational difference analysis and in a primary cultured hepatocyte system. The expression levels of PC3 / BTG2 from liver tissues of the rat model were assessed by RT-PCR and Northern blotting. Meanwhile, the expression of BTG2 mRNA in a tissue microarray of HCC was determined by in situ hybridization. RESULTS: The PC3 / BTG2 gene was rapidly induced after 2/3 partial hepatectomy and its expression peaked within 1-2 hours after operation. HPO rapidly induced the expression of the genes c-fos, LRF-1, and PC3 in primary cultured rat hepatocytes, which might be one of the molecular mechanisms by which HPO stimulates hepatocyte proliferation. Positive BTG2 mRNA expression was detected in 71.19% (42/59) of the HCC samples and in 75% (3/4) of the normal liver tissue samples obtained from the region around the HCC tissues. PC3 / BTG2 mRNA was located mainly in the cytoplasm of HCC cells and its expression was related to the degree of differentiation. CONCLUSIONS: Recombinant human HPO and partial hepatectomy rapidly induce the expression of the PC3 / BTG2 gene. PC3 / BTG2 mRNA is highly expressed in HCC cells and its expression is related to the degree of cell differentiation. The abnormal expression of PC3 / BTG2 is closely related to the genesis and development of HCC, so PC3 / BTG2 may play an important role in these processes.