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目的 :探索脾酪氨酸激酶Syk基因表达与乳腺癌生成及转移的关系 ,以及与雌激素受体 (estrogenrecep tor,ER)、孕激素受体 (progestogenreceptor,PR)、p5 3、HER2 neu的关系。方法 :用半定量RT PCR检测 4 0例乳腺癌标本、正常乳腺组织及其 15例良性乳房纤维瘤组织中SykmRNA的表达 ,并用免疫组化方法检测 4 0例乳腺癌组织中ER、PR、p5 3、HER2 neu的表达的情况。结果 :所有正常乳腺组织都有Syk基因的表达 ,而 4 0例乳腺癌组织中只有 9例表达 ,正常乳腺与乳腺癌组织中Syk基因表达率有显著差异 (χ2 =4 7 4 ,P <0 0 5 ) ,且乳腺癌组织中SykmRNA含量比正常乳腺组织显著降低 (t=3 4 1,P <0 0 5 )。有淋巴结转移的 18例乳腺癌组织中 ,1例有Syk基因表达 ,有淋巴结转移的乳腺癌SykmRNA的表达率和表达水平显著降低 (χ2 =3 77,P <0 0 5 ,t=2 74 ,P <0 0 5 )。结论 :Syk基因的表达在抑制乳腺癌的生长及转移过程中可能起着重要的作用。
OBJECTIVE: To explore the relationship between the expression of Syk gene and the genesis and metastasis of breast cancer, as well as the relationship with estrogen receptor (ER), progestogen receptor (PR), p5 3 and HER2 neu . Methods: The expression of Syk1 mRNA was detected by semi-quantitative RT-PCR in 40 breast cancer specimens, normal breast tissues and 15 benign breast fibroma tissues. The expressions of ER, PR and p5 in 40 breast cancer tissues were detected by immunohistochemistry 3, the expression of HER2 neu. RESULTS: The expression of Syk gene was detected in all normal breast tissues, while only 9 of 40 breast cancer tissues were expressed. The expression of Syk gene in normal breast and breast cancer tissues was significantly different (χ2 = 474, P <0 0 5), and the expression of SykmRNA in breast cancer tissues was significantly lower than that in normal breast tissues (t = 34 1, P <0 05). In 18 cases of breast cancer with lymph node metastasis, Syk gene expression was found in 1 case and SykRNA expression was significantly decreased in breast cancer with lymph node metastasis (χ2 = 3 77, P <0 05, t = 2 74, P <0 0 5). Conclusion: Syk gene expression may play an important role in inhibiting the growth and metastasis of breast cancer.