Effects of Compound Zhebei Granule(复方浙贝颗粒) Combined with Doxorubicin on Expression of Specific Surfa

• 作 者: ZHANG Yu HOU li CHEN Xin-yi
• 机 构: Department of Integrated Chinese and Western Medicine,Tianjin Medical University Cancer Institute an
• 出 处: 中国结合医学杂志（英文版）
• 发表日期: 2018年3期
• 关键词: acute myeloid leukemia Chinese medicine doxorubicin adriamycin

Objective:To investigate the effects of Compound Zhebei Granule(复方浙贝颗粒, CZG) combined with doxorubicin hydrochloride (adriamycin,ADM) on specific surface antigens in mice with KG-1a transplanted cells.Methods:A subcutaneous tumor xenograft model was established by injection of the acute myeloid leukemia cell line KG-1a into the axillary flanks of BALB/c-nude mice.Twenty-four tumor bearing mice were divided int0 4 groups according to a random number table,including normal saline control group,ADM group,high-dose CZG group,and mid-dose CZG group,with 6 mice in each group.Drug administration occurred on the 14th day after cell inoculation,and normal saline control group mice were gavaged with normal saline at 0.2 mL/10 g every other day.ADM group mice were intraperitoneally injected with ADM 1 mg/kg [conversion of adults,40 mg/(m2·d)] every other day.High-and mid-dose CZG groups mice were gavaged with CZG at the dose of 8 and 4 g/kg respectively every other day and intraperitoneally injected with ADM (1 mg/kg) every other day.The administration period lasted for 10 days.The tumor xenografts were made into mononuclear cell suspensions after dissection,and the expressions of specific surface antigens,including CD34+CD38-,CD34+CD38-CD123+,CD34+CD38-CD96+ and CD34+CD38-CD33+,in KG-1a cell line tumor xenografts were detected by flow cytometry.Results:Compared with the control and ADM groups,expression of CD34+CD38-in the two CZG groups was significantly lower (P＜0.05).Compared with the control group,expression of CD34+CD38-CD123+ in the two CZG groups decreased (P＜0.05).The high-dose CZG group showed more obvious outcomes compared with the ADM group (P＜0.05).Compared with the control and ADM groups,the expression of CD34+CD38-CD96+ and CD34+CD38-CD33+ in the two CZG groups decreased (P＜0.05).Conclusions:CZG combined with doxorubicin could reduce the expreseion of CD34+CD38-,CD34+CD38-CD123+,CD34+CD38-CD96+ and CD34+CD38-CD33+ in KG-1a cell line tumor xenografts,which shows that CZG could target leukemia stem cells and play a role in chemosensitization.