Effects of Banxia Xiexin Decoction（半夏泻心汤）on Cisplatin-Induced Apoptosis of Human A549 Lung Cancer Ce

• 作 者: Ha-Rim Kim Guem-San Lee Mi-Seong Kim Do-Gon Ryu Hong-Seob So Hyoung-Chul Moon Young-Rae Lee Sei-Hoon Yang Kang-Beom Kwon
• 机 构: Center for Metabolic Function Regulation, Wonkwang University School of Medicine, Iksan, Jeonbuk 545
• 出 处: 中国结合医学杂志（英文版）
• 发表日期: 2018年6期
• 关键词: Banxia Xiexin Decoction cisplatin A549 cells apoptosis anticancer synergism Chin

Objective:To examinie the synergistic effects of Banxia Xiexin Decoction（半夏泻心汤,Known as Banhasasim-tang in Korean) extract (BXDE) on cisplatin-induced cytotoxicity in the A549 human lung cancer cell lines.Methods:A549 cells were treated with varying concentrations (50-200 μ g/mL) of cisplatin and BXDE alone or in combination for 96 h.We used 1-(4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan assay and flow cytometry to analyze cell viability and apoptosis,respectively.Results:The exposure of cells to cisplatin and BXDE alone or in combination decreased cell viability dose-and time-dependently (P＜0.05),which was found to be mediated by the apoptotic pathway as confirmed by the increase in the annexin V+/propidium iodide stained cell population and a ladder pattem of discontinuous DNA fragments.Furthermore,the apoptosis was inhibited by the pan-caspase inhibitor,benzyloxycarbonyI-VaI-Ala-Asp (OMe) fluoromethylketone (z-VAD-FMK).Conclusions:BXDE significantly potentiated apoptotic effects of cisplatin in A549 cells.Moreover,apoptosis induced by BXDE might be the pivotal mechanism mediating its chemopreventative action against cancer.