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目的利用PVG(TR-1c)和DA(RT-1a)大鼠分别为供受体,联合进行辅助性肝移植和异位心脏移植,建立大鼠辅助性肝移植诱导免疫耐受模型。方法辅助性肝移植采用重建门静脉法,异位心脏移植采用腹腔吻合法。结果同品系对照组和实验组动物及其移植心脏存活超过100 d,而异品系对照组移植心脏只存活7 d。结论在受体肝脏存在的情况下,辅助性供肝依然发挥其诱导耐受的效应。经长期观察,供肝无萎缩,耐受诱导效应持续存在,指示心脏搏动良好。因此,该模型在肝移植基础研究中具有实用价值。
OBJECTIVE: To establish a model of immune tolerance induced by adjuvant liver transplantation in rats by using donor-recipient (PVG) and DA (RT-1a) rats in combination with donor liver transplantation and ectopic heart transplantation respectively. Methods Liver transplantation assisted reconstruction of the portal vein method, heterotopic heart transplantation using abdominal anastomosis. Results The survivals of the same control group and experiment group and their transplanted hearts survived for more than 100 days, while those of the control group of the same strain only survived for 7 days. Conclusions In the presence of recipient liver, the helper donor liver still exerts its effect of inducing tolerance. After long-term observation, there is no atrophy of the liver, and the tolerance-inducing effect persists, indicating that the heart beats well. Therefore, the model has practical value in the basic research of liver transplantation.