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全球哮喘患者人数众多,而且这一数字仍在不断增长.近年来大家把目光聚焦到人造化学物质与哮喘增加的关系上.邻苯二甲酸二乙基己酯(di-(2-ethylhexyl)phthalate,DEHP)是现代社会一种常见的污染物,在室内环境中也有广泛的存在.本研究通过建立卵清白蛋白(OVA)致敏的大鼠哮喘模型,来探讨DEHP对哮喘的影响.将Wistar大鼠随机分为6组,分别为生理盐水对照和两种浓度(0.5mg.m-3和5mg.m-3)的DEHP染毒的无OVA致敏组,以及这三者对应的OVA致敏组.行肺部滴注法对大鼠进行染毒,每天1次,染毒剂量相当于在0.5mg.m-3和5mg.m-3浓度的DEHP中暴露8h.处理后的大鼠通过肺功能检测,ELISA检测肺组织细胞因子和肺组织切片等方法研究其哮喘症状.结果显示DEHP染毒的OVA致敏组具有更显著的哮喘症状.
There are a large number of asthmatics around the world and this number is still growing and in recent years everyone has focused on the relationship between man-made chemicals and increased asthma. Di- (2-ethylhexyl) phthalate , DEHP) is a common pollutant in modern society and also exists widely in indoor environment.In this study, the effect of DEHP on asthma was investigated by establishing a rat asthma model induced by ovalbumin (OVA) .The Wistar Rats were randomly divided into 6 groups: saline-free and DEHP-exposed OVA-sensitized groups at two concentrations (0.5 mg.m-3 and 5 mg.m-3), respectively, and OVA-induced Min group.The rats were inoculated by pulmonary instillation once a day and exposed to DEHP of 0.5mg.m-3 and 5mg.m-3 for 8h.The treated rats The asthma symptoms were studied by pulmonary function tests, ELISA detection of lung tissue cytokines and lung tissue sections, and the results showed that the OVA sensitized group with DEHP had more significant asthma symptoms.