论文部分内容阅读
目的通过对严重急性呼吸综合征(SARS)患者脾内白细胞介素6(IL-6),干扰素β(IFN-β)和干扰素γ诱导蛋白10(IP-10)的免疫组织化学结果分析,深入探讨SARS患者脾组织的病理变化及其发病机制。方法通过免疫组织化学技术观察6例SARS死亡患者脾和6例意外死亡者正常脾组织细胞内IL-6I、FN-β和IP-10的表达情况,并进行图像分析。结果SARS患者脾红髓内含大量IL-6阳性细胞,阳性产物呈团块状分布于细胞质和细胞核。图像分析结果显示,SARS患者脾红髓内IL-6阳性细胞的平均吸光度值(AA)与正常对照组比较,有显著性差异(P<0.05);SARS患者脾红髓内阳性细胞的细胞核、细胞质呈IFN-β阳性,阳性产物呈棕黄色团块状,图像分析结果显示,SARS患者脾组织内IFN-β阳性细胞的AA值与正常对照组比较,有显著性差异(P<0.05);SARS患者脾红髓内含大量IP-10阳性细胞,阳性产物呈棕黄色团块状,分布于细胞核和细胞质。图像分析结果显示,SARS患者脾组织内IP-10阳性细胞的AA值与正常对照组比较,有显著性差异(P<0.05)。3种细胞因子在SARS患者残存的脾白髓内均呈阴性。结论SARS患者脾内细胞的IL-6I、FN-β和IP-10反应均相对增强,提示SARS患者脾组织的病理性损伤以及免疫功能缺陷可能与上述促炎症因子、免疫抑制因子及趋化因子的相对增多有关。
Objective To investigate the immunohistochemical results of interleukin-6 (IL-6), interferon-β (IFN-β) and interferon gamma-inducible protein 10 (IP-10) in spleen of patients with severe acute respiratory syndrome , In-depth study of pathological changes of spleen in SARS patients and its pathogenesis. Methods The expression of IL-6I, FN-β and IP-10 in normal spleen cells from 6 patients with SARS death and 6 patients with accidental death were observed by immunohistochemistry and analyzed by image analysis. Results Serum IL-6 positive cells were found in the spleen red blood cells of SARS patients. The positive products were distributed in cytoplasm and nucleus. The results of image analysis showed that the average absorbance value (AA) of IL-6 positive cells in spleen red blood cells of SARS patients was significantly different from that of the normal control group (P <0.05). The nuclei, The results of image analysis showed that the AA value of IFN-β positive cells in spleen tissue of SARS patients was significantly different from that of normal control group (P <0.05). SARS patients spleen red pulp contains a large number of IP-10 positive cells, the positive product was brown yellow mass, located in the nucleus and cytoplasm. The results of image analysis showed that the AA value of IP-10 positive cells in spleen tissue of SARS patients was significantly different from that of the normal control group (P <0.05). The three kinds of cytokines were all negative in splenic white marrow that remained in SARS patients. Conclusions The splenic cells in SARS patients have relatively increased IL-6I, FN-β and IP-10 responses, suggesting that pathological injury and immune dysfunction may be related to the above-mentioned proinflammatory cytokines, immunosuppressive factors and chemokines Related to the relative increase.