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中耳渗出液的多样性提示了其复杂的发病机理。不论其病因属何种类型,其结果多因血管通透性增加引起中耳渗出液的积聚。因此关于血管活性物质如各类炎性介质或神经肽在中耳渗液产生中的作用也就日益受到人们的注意。作者将炎性介质和标记伊文氏蓝的白蛋白溶液从大鼠尾静脉注入,20分钟切开鼓室。使用50μl 甲酰胺收集中耳液体并再稀释至1.0ml,然后采用双光素分光光度计测定伊文氏蓝-甲酰胺混合物的最大吸光度并同时观察鼓室各部位的粘膜蓝染程度,以此来判断各种炎性介质和神经肽对中耳渗出数量的影响并可推测鼓室
The diversity of middle ear exudates suggests its complex pathogenesis. Irrespective of the type of etiology, the result is mostly the accumulation of exudate in the middle ear due to increased vascular permeability. Therefore, the role of vasoactive substances, such as various types of inflammatory mediators or neuropeptides, in the production of middle ear effusion is also attracting more and more attention. The authors injected inflammatory media and albumin solution labeled with Evans Blue from the tail vein of the rat and the tympanic membrane was dissected for 20 minutes. The middle ear fluid was collected using 50 μl of formamide and diluted to 1.0 ml again, and then the maximum absorbance of the Evans Blue-formamide mixture was measured with a two-photon spectrophotometer and the degree of mucosal staining of mucosa was also observed at each site of the tympanum Effects of various inflammatory mediators and neuropeptides on the number of middle ear exudation and the hypotheses can be inferred