论文部分内容阅读
从采自我国烟草主要产区的38份土壤样品中分离获得放线菌296株.采用平板对峙培养法从中筛选出对烟草青枯病菌(Ralstonia solanacearum)和黑胫病菌(Phytophthora parasitica var.nicotianae)有拮抗活性的菌株227株,其中对青枯病菌拮抗活性较高的YYD3-17、YYD3-19、YYD3-29、YYD3-32和HLL4-9菌株抑菌圈直径达45mm以上;对黑胫病菌拮抗活性较高的BXⅡ3-1、BXⅡ5-4、BXⅡ5-5、BXI21-1和YYD1-4菌株抑菌圈直径达25mm以上.依据前人的研究成果,对这10株放线菌进行初步鉴定,结果显示它们均属于链霉菌属(Strepto-mycesspp.).其中YYD3-17、YYD3-19、YYD3-29和YYD3-32属于灰红紫类群;BXⅡ3-1、BXI21-1同属于吸水类群;而BXⅡ5-4、BXⅡ5-5、YYD1-4和HLL4-9分属白色亚群、白孢亚群、金色亚群和绿色亚群.BXⅡ5-4和BXⅡ5-5分别被鉴定为白色链霉菌种(S.albus)和阿布拉链霉菌种(S.aburviensis).
A total of 296 actinomycetes were isolated from 38 soil samples collected from major tobacco producing areas in China.Among them, Ralstonia solanacearum and Phytophthora parasitica var. Nicotianae were screened out by plate confrontation culture method. 227 strains with antagonistic activities, of which YYD3-17, YYD3-19, YYD3-29, YYD3-32 and HLL4-9 strains with higher antagonistic activity against R. solani were more than 45mm in diameter. The inhibition zone diameters of BXⅡ3-1, BXⅡ5-4, BXⅡ5-5, BXI21-1 and YYD 1-4 strains with higher antagonistic activity were more than 25mm.According to the results of previous studies, the preliminary identification of these 10 strains of actinomycetes The results showed that all of them belonged to Strepto-mycesspp. Among them, YYD3-17, YYD3-19, YYD3-29 and YYD3-32 belonged to the gray purple group; BXⅡ3-1 and BXI21-1 belonged to the same group; While BXⅡ5-4, BXⅡ5-5, YYD1-4 and HLL4-9 belonged to white subgroup, white subunit, golden subgroup and green subgroup.BXⅡ5-4 and BXⅡ5-5 were identified as Streptomyces albus S. albus and S. aburviensis.