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本实验以裸鼠人肝细胞癌动物模型,应用磺化氯铝酞菁(ALSPC)为光敏剂,观察了ALSPC光敏治疗后肿瘤组织的形态学改变。治疗后光镜下见肿瘤细胞明显变性、解离,胞体呈气球样变,胞核固缩,同时毛细血管内皮损伤,瘤组织弥漫性出血。电镜下见部分粗面内质网扩张,线粒体肿胀,核周间隙扩大,最终核染色质凝集,核膜胞膜破裂,细胞死亡。内皮细胞也出现类似改变。提示ALSPC对接种于动物体内的人肝细胞癌有明显的杀伤作用,是一种有效的光敏剂,细胞的膜系统可能是主要作用靶位。
In this experiment, animal models of human hepatocellular carcinoma in nude mice were used. The application of sulfonated aluminum chloroprene phthalocyanine (ALSPC) as a photosensitizer was used to observe the morphological changes of tumor tissue after phototherapy with ALSPC. After treatment, the tumor cells were significantly denatured and dissociated under light microscope. The cell body was balloon-shaped, the nucleus was pyknosis, and the capillary endothelium was damaged. The tumor tissue was diffusely bleeding. Under the electron microscope, part of the rough endoplasmic reticulum expanded, swelling of the mitochondria, expansion of the perinuclear space, eventually agglutination of nuclear chromatin, nuclear membrane membrane rupture, cell death. Similar changes have also occurred in endothelial cells. It is suggested that ALSPC has an obvious killing effect on human hepatocellular carcinoma vaccinated in animals and is an effective photosensitizer. The membrane system of cells may be the main target site.