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近年来许多电生理学工作者用培养的神经细胞研究神经元及突触生理学。这些实验要求神经细胞体外生长良好,发育成熟,接近在体的神经元。选用培养成熟的神经元是实验成败的关键。静息膜电位(膜电位)是神经元发育程度的重要标志。从膜电位水平判断细胞发育状况是保证实验质量的重要条件。现有的电生理实验,没有明确地规定膜电位水平,多数实验中培养神经元的膜电位为-40m.v.——80m.v.,个别实验的神经细膜电位仅有—7.9±22.17m.v.,有些作者选用膜电位为>—40m.v.的神经元做实验对象。损伤的细胞膜电位低于—40m.v.;没有损伤的神经元膜电位超过—40m.v.,是选用的对象。
In recent years, many electrophysiologists have studied neurons and synaptic physiology with cultured nerve cells. These experiments require that neurons grow well in vitro, mature and close to the neurons in the body. Selection of mature neurons is the key to the success of the experiment. Resting membrane potential (membrane potential) is an important indicator of neuronal development. Judging the cell development status from the membrane potential level is an important condition to ensure the quality of the experiment. Existing electrophysiological experiments, there is no clear regulation of the membrane potential level, the majority of cultured neurons in the membrane potential of -40m.v.-- 80m.v., The individual experimental nerve membrane potential of only -7.9 ± 22.17 mv, some authors use the membrane potential of> -40m.v. Damage cell membrane potential below -40m.v .; no damage to the neuron membrane potential over -40m.v., is the object of choice.