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目的了解老年病区肺炎克雷伯菌分离株消毒剂-磺胺耐药基因存在情况和耐药性。方法应用聚合酶链反应法和纸片法对肺炎克雷伯菌消毒剂-磺胺耐药基因和药敏进行了检测。结果该医院老年病区病人标本中分离的80株肺炎克雷伯菌检测出携带消毒剂-磺胺耐药基因63株,检出率为78.8%。分离的80株肺炎克雷伯菌对头孢类、替卡西林和环丙沙星类抗生素耐药率为100%;只对亚胺培南和美罗培南敏感率100%。结论该医院老年病区分离的肺炎克雷伯菌临床株消毒剂-磺胺耐药基因检出率较高,多数对临床常用抗生素耐药,提示临床耐药菌株可能同时对消毒剂存在抗性。
Objective To investigate the presence and drug resistance of Klebsiella pneumoniae isolates in elderly ward. Methods The Klebsiella pneumoniae disinfectant-sulfa resistance gene and drug susceptibility were detected by polymerase chain reaction and disk-blotting. Results Eighty-three strains of Klebsiella pneumoniae isolated from the patient samples of geriatric ward in the hospital detected 63 strains of sulfa drug-resistant genes, the detection rate was 78.8%. 80 isolates of Klebsiella pneumoniae showed a resistance rate of 100% to cephalosporins, ticarcillin and ciprofloxacin antibiotics, and only 100% to imipenem and meropenem. Conclusions The detection rate of sulfanilamide resistance gene in clinical isolates of Klebsiella pneumoniae isolates from geriatric ward of the hospital is high, most of which are resistant to commonly used antibiotics in clinical practice, suggesting that clinical drug-resistant strains may be resistant to disinfectants at the same time.